Deuterium - tagged L - phenylalanine produced strain Brevibacterium methylicum for medicine Print E-mail
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Written by Олег МоÑин   
Sunday, 06 July 2008
The text of the original in Russian 


DEUTERIUM - tagging L-Phenylalanine produced strains Brevibacterium methylicum for medical diagnostics. 


A biosynthesis of deuterium-labeled - L-phenylalanine metilotrofnyh optional strain of bacteria Brevibacterium methylicum, able to assimilate methanol (or deuterium-tagged analogue) as a source of carbon and energy. Biosintez carried out on minimal medium containing 98 vol.% Heavy water and 2% of the deuterium-methanol. Exit L-phenylalanine that was 1 g / liter. Analysis of the degree of L-phenylalanine deyterirovannosti conducted using mass spectrometry electron impact after preparative separation methods of treatment-phase high performance liquid chromatography (HPLC) in the form of methyl ester dance - phenylalanine and karbobenzoksi-phenylalanine. Thus, the selective approach was able to get a strain of bacteria optional metilotrofnyh B. methylicum, adapted to the high content of heavy water in the growth medium. The advantages of this strain to produce deuterium-labeled phenylalanine were improved growth and biosynthetic capacity of the metilotrofa to maximize deuterated medium. By using the strain of B. methylicum able to obtain about 1 gram of deuterium-labeled phenylalanine from 1 L culture medium (phenylalanine has also been isolated from culture fluid B. methylicum treatment-phase HPLC as methyl ester dance-L-phenylalanine to the degree of chromatographic purity of 99% and 89% yield ). According to our data, the degree of isotope incorporation of deuterium in phenylalanine was 75% (6 of hydrogen atoms in the molecule substituted for deuterium), which demonstrates the high efficiency of tagging L-phenylalanine under these conditions. The resulting deyteriymechenny phenylalanine can be used to diagnose inherited phenylketonuria. 

Moscow State Academy of Fine Chemical Technology. MV Lomonosov Moscow State University, 117571 Moscow, prosp. Vernadskogo, d.86. 
     
References 
1. Mosin O.V., Skladnev D.A., Shvets V.I. / / Biosc. biotechnol. biochem. 1998. V. 62. Number 2. P. 225-229. 
2. Mosin OV, Karnauhova EN, Pshenichnikova AB, folding, DA, Akimova OL / / Biotechnology. - 1993. - N. 9. - S. 16-20. 
3. Mosin OV, folding, DA, Shvets VI / / Boorganicheskaya chemistry. - 1996. - N. 3  
 

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