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Science Science | Natural Method tverdofazovoy adaptation of cells to heavy water
Science Science | Natural Method tverdofazovoy adaptation of cells to heavy water
| Method tverdofazovoy adaptation of cells to heavy water |
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| Written by Олег МоÑин | |
| Sunday, 06 July 2008 | |
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The text of the original in Russian Method tverdofazovoy adaptation of cells to heavy water We investigate the adaptation to heavy water bacterial strains-producers of amino acids, proteins and nucleoside of different taxonomic genera of microorganisms, and optional metilotrofnye obligate bacterium Brevibacterium methylicum VKPM In 5652 and Methylobacillus flagellatum CT, bacteria Bacillus subtilis B-3157i galofilnye bacterium Halobacterium halobium ET 1001. Our proposed method of solid-phase adaptation consists of a series of multiple passages of adaptation of cells in a petri dish of bacteria on solid media agarizovannymi step increases the concentration of heavy water in them (from 0 to 98% heavy water). At the same time on a petri dish has been selected individual colonies grown on media containing low concentrations of heavy water. Then their consecutively peresevali on solid medium with a greater degree deyterirovannosti, including the environment with 98% heavy water (degree of survival of bacteria at maximum tyazhelovodorodnoy environment was no more than 50%). Thus in a petri dish with a maximum concentration of heavy water have been allocated individual cell clones, each of which represented the progeny of a single cell, resistant to heavy water. Then they peresevali on the environment with greater deyterirovannosti, including the environment with 98% heavy water (degree of survival of bacteria at maximum tyazhelovodorodnoy environment was no more than 50%). Over the course of adaptation of cells observed on the changes of log-phase, time of cell generation and output of microbial biomass, as well as the maximum level of accumulation of end products of biosynthesis in the culture fluid. The growth of bacteria was evaluated for ability to form individual colonies on the surface of solid agarizovannyh environments, as well as the largest optical density of suspensions of cells measured on the spectrophotometer Beckman-DU6 (USA) at 540 nm. As a result, the proposed method was able to get a fully deuterated cells of microorganisms that can live in environments with a maximum concentration of heavy water Moscow State Academy of Fine Chemical Technology. MV Lomonosov Moscow State University, 117571 Moscow, prosp. Vernadskogo, 86. References: 1. Mosin OV, folding DA, Shvets VI / / Biotechnology. - 2001. - N12. - S. 21-32. 2. Mosin OV, folding DA, Egorova TA, Yurkevich AM, Shvets VI / / Biotechnology. - 1996. - N 3. - S. 3-12. 3. Mosin OV, folding DA, Egorova TA, Shvets VI / / Bioorganic Chemistry. - 1996. - V. 22. - N 10-11. - S. 856-869. |